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Image Search Results
Journal: Frontiers in Microbiology
Article Title: A Functional Slow Recycling Pathway of Transferrin is Required for Growth of Chlamydia
doi: 10.3389/fmicb.2010.00112
Figure Lengend Snippet: Analysis of host cell trafficking pathways during treatment with FR179254 . HEp-2 cells were treated as indicated, and trafficking pathways were analyzed as described in Section (A) Lipid droplet (LD) visualization with the neutral lipid stain Bodipy 493. (B) Fluid-phase uptake as measured by fluorescent dextran. (C) Live cell visualization of multivesicular bodies/late endosomes using CD63-GFP expressing HEp-2 cells. (D) Analysis of late endosomes/lysosomes in LAMP1-YFP transfected cells. (E) Exocytic trafficking as measured by incorporation of fluorescent sphingomyelin (SM) in Golgi and chlamydiae. (F) Exocytic trafficking of glycoproteins as measured by localization of CD4 on the surface of transfected cells. (G,H) Analysis of retrograde trafficking using fluorescently tagged cholera toxin subunit B (CTxB) after pulse-chase labeling. Arrows within (C) and (E) indicate inclusions. Note the lack of significant staining in (C) (inset) and the presence of staining in (E) , which is contrast to a published study (Beatty, ).
Article Snippet: For CD4, non-permeabilized cells were incubated with primary
Techniques: Staining, Expressing, Transfection, Pulse Chase, Labeling
Journal: BJC Reports
Article Title: Circulating extracellular vesicles containing S100A9 reflect histopathology, immunophenotype and therapeutic responses of liver metastasis in colorectal cancer patients
doi: 10.1038/s44276-023-00007-9
Figure Lengend Snippet: a Increased CD4 positive cell expression in interface region of the Desmoplastic Lesion. b Increased CD8 positive cell expression in interface region of the desmoplastic lesion.
Article Snippet: Briefly, slides were Incubated overnight at 4 °C with primary antibody anti-S100A9 (TFS, Catalogue PA5-82145, dilution 1:8000),
Techniques: Expressing